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Optics and Spectroscopy, 2020, Volume 128, Issue 6, Pages 790–794
DOI: https://doi.org/10.21883/OS.2020.06.49412.50-20 (Mi os401) 		 
Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods
Biophotonics

Multiphoton excitation microscopy for identification and operational control of extracellular matrix components of body tissues

Yu. V. Kistenevab, V. V. Nikolaevac, A. V. Borisovab, O. B. Zaevaa, A. I. Knyazkovaac, N. A. Krivovaa

a Tomsk State University
b Siberian State Medical University
c Institute of Strength Physics and Materials Science, Siberian Branch of the Russian Academy of Sciences, Tomsk, Russia
Full-text PDF (4530 kB)
DOI: https://doi.org/10.21883/OS.2020.06.49412.50-20
Abstract: The process of extraction of the extracellular matrix (ECM) requires operational monitoring, since there is a risk of destruction of the main proteins of the cell matrix at non-optimal parameters of the process. Using multiphoton microscopy the structure of collagen, the integrity of the cell membrane and the content of elastin in the process of isolation of ECM from various tissues of the rat body (brain, dermis, and muscle) has been studied. It has been shown that multiphoton microscopy is an effective tool for monitoring the quality of ECM extraction.
Keywords: multiphoton microscopy, extracellular matrix, collagen.
Funding agency Grant number
Program of fundamental scientific research of the State Academies of Sciences III.23
Program for Competitiveness Enhancement of Tomsk State University 8.1.11.2019
This work was carried out as part of the Program of Fundamental Scientific Research of State Academies of Sciences for 2013–2020, direction III.23, and the scientific project 8.1.11.2019, carried out with the support of the TSU competitiveness improvement program.
Received: 11.12.2019
Revised: 12.02.2020
Accepted: 28.02.2020
English version:
Optics and Spectroscopy, 2020, Volume 128, Issue 6, Pages 794–798
DOI: https://doi.org/10.1134/S0030400X20060107
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: Yu. V. Kistenev, V. V. Nikolaev, A. V. Borisov, O. B. Zaeva, A. I. Knyazkova, N. A. Krivova, “Multiphoton excitation microscopy for identification and operational control of extracellular matrix components of body tissues”, Optics and Spectroscopy, 128:6 (2020), 790–794; Optics and Spectroscopy, 128:6 (2020), 794–798
Citation in format AMSBIB
\Bibitem{KisNikBor20}
\by Yu.~V.~Kistenev, V.~V.~Nikolaev, A.~V.~Borisov, O.~B.~Zaeva, A.~I.~Knyazkova, N.~A.~Krivova
\paper Multiphoton excitation microscopy for identification and operational control of extracellular matrix components of body tissues
\jour Optics and Spectroscopy
\yr 2020
\vol 128
\issue 6
\pages 790--794
\mathnet{http://mi.mathnet.ru/os401}
\crossref{https://doi.org/10.21883/OS.2020.06.49412.50-20}
\elib{https://elibrary.ru/item.asp?id=43808063}
\transl
\jour Optics and Spectroscopy
\yr 2020
\vol 128
\issue 6
\pages 794--798
\crossref{https://doi.org/10.1134/S0030400X20060107}
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