This article is cited in 2 scientific papers (total in 2 papers)
Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods Biophotonics
Nuclear magnetic resonance and infrared spectroscopy examination of blood for diagnosis of the diffuse hepatopathy state
Abstract:
67 middle-aged (48 ± 1 years) patients with diffuse liver disease of various origins have been examined. It has been established that an increase in the degree of fibrosis is associated with an increase in the resonance intensity of glucose-6-phosphates, adenosine monophosphate (AMP), inosine monophosphate (IMP), pyrophosphates, and 2,3-bisphosphoglyceric acid (BPG) and a decrease in the resonances of inorganic phosphates and phosphocreatine, γ-, α-, and β-AMP, uridine triphosphate (UTP), Hb- and Mg-adenosine triphosphate (ATP), and β- and α- adenosine diphosphate (ADP). The predictive value of a positive result of 31Р NMR spectroscopy of erythrocytes for liver fibrosis is 92.3% (the sensitivity is 85.71% and the specificity is 80%). In the IR spectra of the blood serum of patients with severe fibrosis, in contrast to the cases of moderate fibrosis, the resonance intensity is higher at 1280 and 3190 cm−1 and significantly lower at 776, 818, 889, 966, 1399, 1450, 1570, and 1635 cm−1, which indicates the presence of protein molecules with a secondary structure mainly in the form of a spiral (α helix), in contrast to the structure in the form of a folded sheet (β sheet) at p< 0.001–0.05. The combined use of 31Р NMR spectroscopy of erythrocyte suspensions and IR spectroscopy of blood serum made it possible to increase the diagnostic accuracy (96%), sensitivity (97%), and specificity (91%) in distinguishing the degrees of liver fibrosis as compared with the separate use of these techniques.
Keywords:31Р NMR spectroscopy, IR spectroscopy, erythrocytes, blood serum, diffuse hepatopathy, degree of fibrosis.
This study was carried out under the theme “Epidemiological Monitoring of the State of Public Health and Study of the Molecular Genetic and Molecular Biological Mechanisms of the Development of Common Therapeutic Diseases in Siberia for Improving Approaches to Their Diagnosis, Prevention, and Treatment”, state assignment no. 0324-2018-0001, reg. no. АААА-А17-117112850280-2.
Citation:
M. V. Kruchinina, V. N. Kruchinin, G. V. Shuvalov, I. V. Minin, O. V. Minin, “Nuclear magnetic resonance and infrared spectroscopy examination of blood for diagnosis of the diffuse hepatopathy state”, Optics and Spectroscopy, 128:6 (2020), 783–789; Optics and Spectroscopy, 128:6 (2020), 787–793
M. V. Kruchinina, A. A. Gromov, L. V. Shcherbakova, E. V. Kruchinina, V. M. Generalov, K. V. Generalov, V. N. Kruchinin, S. V. Rykhlitskii, I. N. Yakovina, M. V. Yakovlev, O. V. Minin, I. V. Minin, “Electrical and viscoelastic parameters of red blood cells in models for the diagnosis of adenomatous polyps and stages of colorectal cancer with optical detection of cells in non-uniform alternating electric field”, Optics and Spectroscopy, 129:12 (2021), 1327–1340