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This article is cited in 7 scientific papers (total in 7 papers)
Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods
Biophotonics
Cellular uptake study of antimycotic-loaded carriers using imaging flow cytometry and confocal laser scanning microscopy
R. A. Verkhovskii, E. V. Lengert, M. S. Savelyeva, A. A. Kozlova, V. V. Tuchin, Yu. I. Svenskaya Saratov State University
Abstract:
The development and optimization of remote drug delivery systems is a flourishing branch of pharmacy. There is a number of requirements for such systems, among which are biocompatibility and, in some cases, the efficiency of intracellular delivery. The cellular survival after the carrier entrapment is an indirect evidence of biocompatibility of the applied system. The internalization process, as well as main characteristics of carriers themselves can be investigated by imaging flow cytometry and confocal laser scanning microscopy. However, the protocols of carriers' characterization and the assessment of their uptake efficiency are needed to be improved. In this work, we optimized the evaluation technic for particles' internalization process by imaging flow cytometry. The confocal laser scanning microscopy was applied as a control method of the particle uptake investigation. The comparative research showed a good correlation between the obtained data. Thus, the high throughput capability of imaging flow cytometry together with the optimized technic of the determination of particle localization inside the cell will further allow us to estimate faster the internalization efficiency of the object under investigation.
Keywords:
imaging flow cytometry, confocal laser scanning microscopy, targeted drug delivery, submicron containers, estimation of cellular uptake.
Received: 12.12.2019 Revised: 27.01.2020 Accepted: 28.02.2020
Citation:
R. A. Verkhovskii, E. V. Lengert, M. S. Savelyeva, A. A. Kozlova, V. V. Tuchin, Yu. I. Svenskaya, “Cellular uptake study of antimycotic-loaded carriers using imaging flow cytometry and confocal laser scanning microscopy”, Optics and Spectroscopy, 128:6 (2020), 795–804; Optics and Spectroscopy, 128:6 (2020), 799–808
Linking options:
https://www.mathnet.ru/eng/os402 https://www.mathnet.ru/eng/os/v128/i6/p795
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