This article is cited in 9 scientific papers (total in 9 papers)
Saratov Fall Meeting 19: 7th International Symposium ''Optics and Biophotonics'', 23d International School for Junior Scientists and Students on Optics, Laser Physics & Biophotonics and 4th School on Advanced Fluorescence Imaging Methods Biophotonics
Cellular uptake study of antimycotic-loaded carriers using imaging flow cytometry and confocal laser scanning microscopy
Abstract:
The development and optimization of remote drug delivery systems is a flourishing branch of pharmacy. There is a number of requirements for such systems, among which are biocompatibility and, in some cases, the efficiency of intracellular delivery. The cellular survival after the carrier entrapment is an indirect evidence of biocompatibility of the applied system. The internalization process, as well as main characteristics of carriers themselves can be investigated by imaging flow cytometry and confocal laser scanning microscopy. However, the protocols of carriers' characterization and the assessment of their uptake efficiency are needed to be improved. In this work, we optimized the evaluation technic for particles' internalization process by imaging flow cytometry. The confocal laser scanning microscopy was applied as a control method of the particle uptake investigation. The comparative research showed a good correlation between the obtained data. Thus, the high throughput capability of imaging flow cytometry together with the optimized technic of the determination of particle localization inside the cell will further allow us to estimate faster the internalization efficiency of the object under investigation.
Keywords:
imaging flow cytometry, confocal laser scanning microscopy, targeted drug delivery, submicron containers, estimation of cellular uptake.
Citation:
R. A. Verkhovskii, E. V. Lengert, M. S. Savelyeva, A. A. Kozlova, V. V. Tuchin, Yu. I. Svenskaya, “Cellular uptake study of antimycotic-loaded carriers using imaging flow cytometry and confocal laser scanning microscopy”, Optics and Spectroscopy, 128:6 (2020), 795–804; Optics and Spectroscopy, 128:6 (2020), 799–808
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\by R.~A.~Verkhovskii, E.~V.~Lengert, M.~S.~Savelyeva, A.~A.~Kozlova, V.~V.~Tuchin, Yu.~I.~Svenskaya
\paper Cellular uptake study of antimycotic-loaded carriers using imaging flow cytometry and confocal laser scanning microscopy
\jour Optics and Spectroscopy
\yr 2020
\vol 128
\issue 6
\pages 795--804
\mathnet{http://mi.mathnet.ru/os402}
\crossref{https://doi.org/10.21883/OS.2020.06.49413.26-20}
\elib{https://elibrary.ru/item.asp?id=43808064}
\transl
\jour Optics and Spectroscopy
\yr 2020
\vol 128
\issue 6
\pages 799--808
\crossref{https://doi.org/10.1134/S0030400X20060235}
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This publication is cited in the following 9 articles:
Polina A. Demina, Mariia S. Saveleva, Roman A. Verkhovskii, Roman A. Anisimov, Pavel S. Pidenko, Yulia I. Svenskaya, “Investigation and Release Profile Optimization for Vaterite-Based Carriers Loaded with Betamethasone Dipropionate”, BioNanoSci., 15:1 (2025)
Anatolii A. Abalymov, Roman A. Anisimov, Polina A. Demina, Veronika A. Kildisheva, Alexandra E. Kalinova, Alexey A. Serdobintsev, Nadezhda G. Novikova, Dmitry B. Petrenko, Alexandr V. Sadovnikov, Denis V. Voronin, Maria V. Lomova, “Time-Delayed Anticancer Effect of an Extremely Low Frequency Alternating Magnetic Field and Multimodal Protein–Tannin–Mitoxantrone Carriers with Brillouin Microspectroscopy Visualization In Vitro”, Biomedicines, 12:2 (2024), 443
Mariia S. Saveleva, Roman A. Verkhovskii, Polina A. Demina, Yury I. Surkov, Roman A. Anisimov, Ekaterina S. Prikhozhdenko, Pavel S. Pidenko, Isabella A. Serebryakova, Sergey M. Zaytsev, Valery V. Tuchin, Yulia I. Svenskaya, “Biodegradable calcium carbonate carriers for the topical delivery of clobetasol propionate”, J. Mater. Chem. B, 12:20 (2024), 4867
Ahmad Saleh, Daniel Stengel, Martyna Truszkowska, Mariana Blanco Massani, Gergely Kali, Andreas Bernkop-Schnürch, “Nanostructured lipid carriers decorated with polyphosphate coated linear and loop cell-penetrating peptides”, International Journal of Pharmaceutics, 2024, 124844
Roman A. Verkhovskii, Anastasiia A. Kozlova, Ekaterina V. Lengert, Mariia S. Saveleva, Mikhail A. Makarkin, Artem M. Mylnikov, Nikita A. Navolokin, Alla B. Bucharskaya, Georgy S. Terentyuk, Ilya A. Bosak, Natalya V. Vasilyeva, Yulia I. Svenskaya, “Cytotoxicity, Dermal Toxicity, and In Vivo Antifungal Effect of Griseofulvin-Loaded Vaterite Carriers Administered via Sonophoresis”, ACS Infect. Dis., 9:5 (2023), 1137
Yulia Svenskaya, Tatiana Pallaeva, “Exploiting Benefits of Vaterite Metastability to Design Degradable Systems for Biomedical Applications”, Pharmaceutics, 15:11 (2023), 2574
Mariia S. Saveleva, Ekaterina V. Lengert, Roman A. Verkhovskii, Anatolii A. Abalymov, Anton M. Pavlov, Alexey V. Ermakov, Ekaterina S. Prikhozhdenko, Sergei N. Shtykov, Yulia I. Svenskaya, “CaCO3-based carriers with prolonged release properties for antifungal drug delivery to hair follicles”, Biomater. Sci., 10:12 (2022), 3323
Roman Verkhovskii, Alexey Ermakov, Oleg Grishin, Mikhail A. Makarkin, Ilya Kozhevnikov, Mikhail Makhortov, Anastasiia Kozlova, Samia Salem, Valery Tuchin, Daniil Bratashov, “The Influence of Magnetic Composite Capsule Structure and Size on Their Trapping Efficiency in the Flow”, Molecules, 27:18 (2022), 6073
M. S. Savelyeva, E. V. Lengert, A. M. Abramova, S. N. Shtykov, Yu. I. Svenskaya, “Spectroscopic study of the release kinetics of water-insoluble drug griseofulvin from vaterite containers in aqueous medium”, Optics and Spectroscopy, 129:7 (2021), 813–820
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