Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides

Disaccharides with the terminal Glcβ1-3 motif were synthesized as probes for studying human blood antibodies. An antibody isolated using Glcβ1-3GalNAcα–Sepharose was found to bind the inner part of the polysaccharide [–4GlcA6LThr3Acβ1-6Galβ1-6Glcβ1-3GalNAc6Acβ1–]_n as evidenced by the use of a printed glycan array and inhibition assays.