|
This article is cited in 1 scientific paper (total in 1 paper)
Physics
The influence of urea on G-quadruplex and i-motif structures in complementary DNA sequences
Y. B. Dalyan, L. G. Aslanyan, I. V. Vardanyan Faculty of Physics, Yerevan State University
Abstract:
In the present study, the methods of circular dichroism and UV/Vis spectrophotometry were used to study the influence of urea on the structural transitions i-motif $\leftrightarrows$ unfolded single strand in cytosine-rich d[3$^{\prime}$-(CCCAAT)$_{3}$CCC-5)$^{\prime}$] region of telomeric DNA (Tel22C) and G-quadruplex $\leftrightarrows$ unfolded single strand in complementary guanine-rich strand d[5$^{\prime}$-A(GGGTTA)$_{3}$GGG-3$^{\prime}$] (Tel22G) at pH 5.5 and 400 mM Na$^+$. Under these conditions, Tel22C and Tel22G were found to form stable i-motif and G-quadruplex structures. It has been shown that urea (0–8 M) destabilizes the i-motif and G-quadruplex structures, but unlike thermal denaturation, it does not destroy the structures completely. The melting processes of G-quadruplex and i-motif are separated in the temperature scale (at any concentration of urea, the melting of the G-quadruplex starts at temperatures where the melting of the i-motif has already been completed.
Keywords:
telomeric DNA, G-quadruplex, i-motif, urea, circular dichroism, UV/Vis spectrophotometry.
Received: 07.07.2020 Revised: 21.07.2020 Accepted: 17.08.2020
Citation:
Y. B. Dalyan, L. G. Aslanyan, I. V. Vardanyan, “The influence of urea on G-quadruplex and i-motif structures in complementary DNA sequences”, Proceedings of the YSU, Physical and Mathematical Sciences, 54:2 (2020), 155–122
Linking options:
https://www.mathnet.ru/eng/uzeru713 https://www.mathnet.ru/eng/uzeru/v54/i2/p155
|
Statistics & downloads: |
Abstract page: | 65 | Full-text PDF : | 15 | References: | 9 |
|