Stepwise Enzymatic Synthesis of the Specific C(3')–C(5') Internucleotide Bond

The review deals with the methods of synthesis of low-molecular-weight oligoribonucleotides based on the use of various ribonucleases and polynucleotide phosphorylase, whereby oligonucleotides can be obtained rapidly and in fairly high yields by a gradual (stepwise) growth of the oligonucleotide chain. The possibilities of this comparatively new field in the synthesis of oligonucleotides and the prospects for its development are discussed.
The bibliography includes 72 references.