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This article is cited in 17 scientific papers (total in 17 papers)
Biosensors for the determination of environmental inhibitors of enzymes
G. A. Evtugyna, H. C. Budnikova, E. B. Nikolskayab a Kazan State University
b I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, St. Petersburg
Abstract:
Characteristic features of functioning and practical application of enzyme-based biosensors for the determination of environmental pollutants as enzyme inhibitors are considered with special emphasis on the influence of the methods used for the measurement of the rates of enzymic reactions, of enzyme immobilisation procedure and of the composition of the reaction medium on the analytical characteristics of inhibitor assays. The published data on the development of biosensors for detecting pesticides and heavy metals are surveyed. Special attention is given to the use of cholinesterase-based biosensors in environmental and analytical monitoring. The approaches to the estimation of kinetic parameters of inhibition are reviewed and the factors determining the selectivity and sensitivity of inhibitor assays in environmental objects are analysed. The bibliography includes 195 references.
Received: 13.04.1999
Citation:
G. A. Evtugyn, H. C. Budnikov, E. B. Nikolskaya, “Biosensors for the determination of environmental inhibitors of enzymes”, Usp. Khim., 68:12 (1999), 1142–1167; Russian Chem. Reviews, 68:12 (1999), 1041–1064
Linking options:
https://www.mathnet.ru/eng/rcr1500https://doi.org/10.1070/RC1999v068n12ABEH000525 https://www.mathnet.ru/eng/rcr/v68/i12/p1142
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