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This article is cited in 1 scientific paper (total in 1 paper)
Second All-Union School on Applications of Lasers in Biology, Tbilisi, November 24-29, 1980
Pulsed fluorometry of primary photosynthesis processes in higher plants
V. Z. Pashchenko, L. B. Rubin
Abstract:
The principles of pulsed fluorometry are presented and an experimental apparatus in which fluorescence is excited by single optical pulses of ~2 psec duration at λ = 530, 353, and 265 nm is described. Fluorescence is recorded using two image-converter cameras with a temporal resolution of 2 and 20 psec. Luminescence from the camera screen is detected by a vidicon and then processed in an analyzer-computer system. The results of experimental investigations of primary photosynthesis processes are presented. It is established that energy transfer within the light-gathering assembly is achieved by localized excitons having the following parameters: diffusion coefficient D~2×10–2 cm2/sec, diffusion length L~90 nm, and transfer probability W~1012 sec–1. Evolution of excitation in focusing antennas of photosystems and long-wavelength pigment–protein complexes is studied. The fluorescence lifetime and quantum yield of photoactive chlorophyll are determined, these being ~60 psec and 3×10–3 (P680) and 5–7 psec and 3×10–4 (P700).
Citation:
V. Z. Pashchenko, L. B. Rubin, “Pulsed fluorometry of primary photosynthesis processes in higher plants”, Kvantovaya Elektronika, 8:12 (1981), 2569–2585 [Sov J Quantum Electron, 11:12 (1981), 1566–1575]
Linking options:
https://www.mathnet.ru/eng/qe8651 https://www.mathnet.ru/eng/qe/v8/i12/p2569
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Abstract page: | 139 | Full-text PDF : | 64 |
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