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This article is cited in 4 scientific papers (total in 4 papers)
Laser biophotonics
Polyacrylamide-based phantoms of human skin for hyperspectral fluorescence imaging and spectroscopy
V. V. Shupletsova, E. A. Zherebtsovab, V. V. Dreminac, A. P. Popovd, A. V. Bykovb, E. V. Potapovaa, A. V. Dunaeva, I. V. Meglinskibcef a Research and Development Centre of Biomedical Photonics, Orel State University
b Optoelectronics and Measurements Techniques Unit, University of Oulu, Finland
c College of Engineering and Physical Sciences, Aston University, UK
d VTT Technical Research Centre of Finland, Oulu, Finland
e National Engineering Physics Institute "MEPhI", Moscow
f I. M. Sechenov First Moscow State Medical University
Abstract:
Based on the combined use of polymerisable polyacrylamide, collagen and an aqueous solution of Flavin adenine dinucleotide (FAD), we have developed a new technique for fabrication of composite phantoms mimicking fluorescence properties of human skin. A comparative analysis of the absorption and scattering coefficients, refractive indices, as well as fluorescence spectra of phantoms with different concentrations of FAD measured with the combined use of a CCD spectrometer and a hyperspectral camera is presented. To obtain values of scattering coefficients close to those for human skin, zinc oxide (ZnO) nanoparticles are added to the polyacrylamide polymer structure. Variations both in the shape of the spectrum and in the intensity of the fluorescence signal in the phantoms are provided by the changes in the volume fraction of FAD and collagen. It is shown that the model fluorescence spectra are in good agreement with the results of direct human skin measurements in vivo.
Keywords:
optical properties, human skin, phantoms, collagen, flavin adenine dinucleotide, hyperspectral imaging, fluorescence spectroscopy.
Received: 21.12.2020
Citation:
V. V. Shupletsov, E. A. Zherebtsov, V. V. Dremin, A. P. Popov, A. V. Bykov, E. V. Potapova, A. V. Dunaev, I. V. Meglinski, “Polyacrylamide-based phantoms of human skin for hyperspectral fluorescence imaging and spectroscopy”, Kvantovaya Elektronika, 51:2 (2021), 118–123 [Quantum Electron., 51:2 (2021), 118–123]
Linking options:
https://www.mathnet.ru/eng/qe17402 https://www.mathnet.ru/eng/qe/v51/i2/p118
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Abstract page: | 156 | Full-text PDF : | 15 | References: | 22 | First page: | 8 |
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