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This article is cited in 5 scientific papers (total in 5 papers)
Laser biophotonics
Prospects for multimodal imaging of biological tissues using fluorescence imaging
D. K. Tuchinaabc, I. G. Meerovichc, O. A. Sindeevaa, V. V. Zherdevac, N. I. Kazachkinac, I. D. Solov'evc, A. P. Savitskyc, A. A. Bogdanov jr.cd, V. V. Tuchinabce a Saratov State University
b Tomsk State University
c Federal Research Centre "Fundamentals of Biotechnology", Russian Academy of Sciences, Moscow
d University of Massachusetts Medical School, Worcester, MA, United States
e Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov
Abstract:
We investigate skin optical clearing in laboratory animals ex vivo and in vivo by means of low-molecular-weight paramagnetic contrast agents used in magnetic resonance imaging (MRI) and a radiopaque agent used in computed tomography (CT) to increase the sounding depth and image contrast in the methods of fluorescence laser imaging and optical coherence tomography (OCT). The diffusion coefficients of the MRI agents Gadovist®, Magnevist®, and Dotarem®, which are widely used in medicine, and the Visipaque® CT agent in ex vivo mouse skin, are determined from the collimated transmission spectra. MRI agents Gadovist® and Magnevist® provide the greatest optical clearing (optical transmission) of the skin, which allowed: 1) an almost 19-fold increase in transmission at 540 nm and a 7 – 8-fold increase in transmission in the NIR region from 750 to 900 nm; 2) a noticeable improvement in OCT images of skin architecture; and 3) a 5-fold increase in the ratio of fluorescence intensity to background using TagRFP-red fluorescent marker protein expressed in a tumour, after application to the skin of animals in vivo for 15 min. The obtained results are important for multimodal imaging of tumours, namely, when combining laser fluorescence and OCT methods with MRI and CT, since the contrast agents under study can simultaneously enhance the contrast of several imaging methods.
Keywords:
optical tomography, OCT, MRI, CT, optical clearing, contrast MRI and CT agents, skin of laboratory animals, in vivo, ex vivo.
Received: 21.12.2020
Citation:
D. K. Tuchina, I. G. Meerovich, O. A. Sindeeva, V. V. Zherdeva, N. I. Kazachkina, I. D. Solov'ev, A. P. Savitsky, A. A. Bogdanov jr., V. V. Tuchin, “Prospects for multimodal imaging of biological tissues using fluorescence imaging”, Kvantovaya Elektronika, 51:2 (2021), 104–117 [Quantum Electron., 51:2 (2021), 104–117]
Linking options:
https://www.mathnet.ru/eng/qe17401 https://www.mathnet.ru/eng/qe/v51/i2/p104
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