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Laser medicine and biology
On contrast of biological X-ray nanomicroscopy
I. A. Artyukov, A. V. Vinogradov, N. L. Popov P. N. Lebedev Physical Institute, Russian Academy of Sciences, Moscow
Abstract:
We analyse the absorption contrast of histological and cytological preparations, which can be achieved in nanomicroscopic studies using monochromatic radiation in the spectral range of 90–600 eV (14–2 nm). Two types of unstained biological objects are considered: untreated and fixed in paraffin, and optimum wavelengths are determined for the study of samples with a thickness of 0.5–10 μm with a spatial resolution of 100–20 nm. Taking into account the efficiency of X-ray optics, the number of source photons required to produce a single image is estimated. It is shown that the greatest interest for the study of fixed objects represents the spectral region of 7–14 nm, for which, on the basis of rapidly developing compact sources of incoherent and coherent radiation and effective optics, microscopes for scientific and clinical research can be designed.
Keywords:
nanomicroscopy, spectral range of 2–14 nm, biological object, exposure dose.
Received: 18.06.2017
Citation:
I. A. Artyukov, A. V. Vinogradov, N. L. Popov, “On contrast of biological X-ray nanomicroscopy”, Kvantovaya Elektronika, 47:11 (2017), 1041–1044 [Quantum Electron., 47:11 (2017), 1041–1044]
Linking options:
https://www.mathnet.ru/eng/qe16713 https://www.mathnet.ru/eng/qe/v47/i11/p1041
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Abstract page: | 184 | Full-text PDF : | 36 | References: | 36 | First page: | 20 |
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