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This article is cited in 13 scientific papers (total in 13 papers)
Extreme light fields and their applications
Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development
A. A. Osychenkoa, A. D. Zalesskiia, A. S. Krivokharchenkoa, A. K. Zhakhbazyana, A. V. Ryabovab, V. A. Nadtochenkoacd a N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow
b A. M. Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow
c Lomonosov Moscow State University, Faculty of Chemistry
d Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region
Abstract:
Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60 %. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo up to the blastocyst stage after exposure of the embryos from different mice to a femtosecond pulse is presented. It is shown that the efficiency of blastocyst formation essentially depends on the biological characteristics of the embryo, namely, the strain and age of the donor mouse. The possibility of obtaining hexaploid embryonal cells using the methods of femtosecond laser surgery is demonstrated.
Keywords:
laser nanosurgery, preimplantation development.
Received: 29.01.2015 Revised: 06.02.2015
Citation:
A. A. Osychenko, A. D. Zalesskii, A. S. Krivokharchenko, A. K. Zhakhbazyan, A. V. Ryabova, V. A. Nadtochenko, “Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development”, Kvantovaya Elektronika, 45:5 (2015), 498–502 [Quantum Electron., 45:5 (2015), 498–502]
Linking options:
https://www.mathnet.ru/eng/qe16170 https://www.mathnet.ru/eng/qe/v45/i5/p498
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