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Kvantovaya Elektronika, 2014, Volume 44, Number 7, Pages 646–651 (Mi qe15988)  

This article is cited in 49 scientific papers (total in 49 papers)

Laser biophotonics

Comparison of in vivo and ex vivo laser scanning microscopy and multiphoton tomography application for human and porcine skin imaging

M. E. Darvina, H. Richtera, Y. J. Zhuba, M. C. Meinkea, F. Knorra, S. A. Gonchukovc, K. Koenigd, J. Lademanna

a Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité – Universitätsmedizin Berlin, Germany
b Zhejiang University of Science and Technology, Hangzhou, China
c National Engineering Physics Institute "MEPhI", Moscow
d JenLab GmbH, Jena, Germany
References:
Abstract: Two state-of-the-art microscopic optical methods, namely, confocal laser scanning microscopy in the fluorescence and reflectance regimes and multiphoton tomography in the autofluorescence and second harmonic generation regimes, are compared for porcine skin ex vivo and healthy human skin in vivo. All skin layers such as stratum corneum (SC), stratum spinosum (SS), stratum basale (SB), papillary dermis (PD) and reticular dermis (RD) as well as transition zones between these skin layers are measured noninvasively at a high resolution, using the above mentioned microscopic methods. In the case of confocal laser scanning microscopy (CLSM), measurements in the fluorescence regime were performed by using a fluorescent dye whose topical application on the surface is well suited for the investigation of superficial SC and characterisation of the skin barrier function. For investigations of deeply located skin layers, such as SS, SB and PD, the fluorescent dye must be injected into the skin, which markedly limits fluorescence measurements using CLSM. In the case of reflection CLSM measurements, the obtained results can be compared to the results of multiphoton tomography (MPT) for all skin layers excluding RD. CLSM cannot distinguish between dermal collagen and elastin measuring their superposition in the RD. By using MPT, it is possible to analyse the collagen and elastin structures separately, which is important for the investigation of anti-aging processes. The resolution of MPT is superior to CLSM. The advantages and limitations of both methods are discussed and the differences and similarities between human and porcine skin are highlighted.
Keywords: dermatology, imaging of human and porcine skin, confocal laser scanning microscopy, multiphoton tomography.
Received: 07.03.2014
Revised: 22.04.2014
English version:
Quantum Electronics, 2014, Volume 44, Issue 7, Pages 646–651
DOI: https://doi.org/10.1070/QE2014v044n07ABEH015488
Bibliographic databases:
Document Type: Article
PACS: 42.62.Be, 87.63.lt, 87.64.mk, 87.57.Q-
Language: Russian


Citation: M. E. Darvin, H. Richter, Y. J. Zhu, M. C. Meinke, F. Knorr, S. A. Gonchukov, K. Koenig, J. Lademann, “Comparison of in vivo and ex vivo laser scanning microscopy and multiphoton tomography application for human and porcine skin imaging”, Kvantovaya Elektronika, 44:7 (2014), 646–651 [Quantum Electron., 44:7 (2014), 646–651]
Linking options:
  • https://www.mathnet.ru/eng/qe15988
  • https://www.mathnet.ru/eng/qe/v44/i7/p646
  • This publication is cited in the following 49 articles:
    Citing articles in Google Scholar: Russian citations, English citations
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