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This article is cited in 8 scientific papers (total in 8 papers)
Extreme light fields and their applications
Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells
I. V. Il'inaa, A. V. Ovchinnikova, O. V. Chefonova, D. S. Sitnikova, M. B. Agranata, A. S. Mikaelyanb a Joint Institute for High Temperatures, Russian Academy of Sciences, Moscow
b Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow
Abstract:
IR femtosecond laser pulses were used for microsurgery of a cell membrane aimed at local and short-duration change in its permeability and injection of specified extracellular substances into the cells. The possibility of noncontact laser delivery of the propidium iodide fluorescent dye and the pEGFP plasmid, encoding the green fluorescent protein, into the cells with preservation of the cell viability was demonstrated.
Keywords:
femtosecond laser pulses, laser microsurgery, optical transfection, cell membrane, optoporation, optoinjection.
Received: 24.12.2012
Citation:
I. V. Il'ina, A. V. Ovchinnikov, O. V. Chefonov, D. S. Sitnikov, M. B. Agranat, A. S. Mikaelyan, “Noncontact microsurgery of cell membranes using femtosecond laser pulses for optoinjection of specified substances into cells”, Kvantovaya Elektronika, 43:4 (2013), 365–369 [Quantum Electron., 43:4 (2013), 365–369]
Linking options:
https://www.mathnet.ru/eng/qe15115 https://www.mathnet.ru/eng/qe/v43/i4/p365
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