Kvantovaya Elektronika
RUS  ENG    JOURNALS   PEOPLE   ORGANISATIONS   CONFERENCES   SEMINARS   VIDEO LIBRARY   PACKAGE AMSBIB  
General information
Latest issue
Archive
Impact factor
Submit a manuscript

Search papers
Search references

RSS
Latest issue
Current issues
Archive issues
What is RSS



Kvantovaya Elektronika:
Year:
Volume:
Issue:
Page:
Find






Personal entry:
Login:
Password:
Save password
Enter
Forgotten password?
Register


Kvantovaya Elektronika, 2012, Volume 42, Number 6, Pages 505–508 (Mi qe14706)  

Biophotonics

Influence of femtosecond laser radiation on cells of the transplantable tumour Krebs-2

Yu. P. Meshalkinab, N. A. Popovabc, V. P. Nikolinc, V. I. Kaledinc, A. V. Kirpichnikova, E. V. Pestryakova

a Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk
b Novosibirsk State Technical University
c Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk
References:
Abstract: The influence of femtosecond radiation of a titaniumsapphire laser on cells of the transplantable ascitic tumour Krebs-2 was studied. After in vitro irradiation by the pulsed fundamentalharmonic radiation with the wavelength 800 nm, pulse duration 30 fs, repetition rate 1 kHz, mean power 100 and 300 mW and exposure time 3 min, as well as by the second-harmonic radiation (40 nm, 50 fs, 120 mW), all cells were diffusely stained by the vital stain trypan blue, which may be an evidence of their death or abnormalities of membrane permeability. However, implantation of such cells to experimental animals led to formation of tumours at the transplantation site with the kinetics slightly different from the control one. In the group of mice to which the cells were inoculated after irradiation with second harmonic pulses of titanium-sapphire laser the inhibition of tumour growth was observed due to partial death of cells under the action of UV spectral components. To explain the mechanism of the observed phenomenon the possibility of pore formation (photoporation) in the cell membrane, described earlier in the papers on foreign DNA transfection into cells, is considered.
Keywords: titanium-sapphire laser, femtosecond radiation, Krebs-2 tumour cells, trypan blue, photoporation, in vivo kinetics of tumour growth.
Received: 12.08.2011
English version:
Quantum Electronics, 2012, Volume 42, Issue 6, Pages 505–508
DOI: https://doi.org/10.1070/QE2012v042n06ABEH014706
Bibliographic databases:
Document Type: Article
PACS: 42.62.Be, 87.50.W-, 87.19.xj, 42.65.Re
Language: Russian


Citation: Yu. P. Meshalkin, N. A. Popova, V. P. Nikolin, V. I. Kaledin, A. V. Kirpichnikov, E. V. Pestryakov, “Influence of femtosecond laser radiation on cells of the transplantable tumour Krebs-2”, Kvantovaya Elektronika, 42:6 (2012), 505–508 [Quantum Electron., 42:6 (2012), 505–508]
Linking options:
  • https://www.mathnet.ru/eng/qe14706
  • https://www.mathnet.ru/eng/qe/v42/i6/p505
  • Citing articles in Google Scholar: Russian citations, English citations
    Related articles in Google Scholar: Russian articles, English articles
    Квантовая электроника Quantum Electronics
    Statistics & downloads:
    Abstract page:213
    Full-text PDF :139
    References:47
    First page:2
     
      Contact us:
     Terms of Use  Registration to the website  Logotypes © Steklov Mathematical Institute RAS, 2024