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This article is cited in 5 scientific papers (total in 5 papers)
Optical technologies in biophysics and medicine
Determination of photophysical parameters of chlorophyll α in photosynthetic organisms using the method of nonlinear laser fluorimetry
T. S. Gostev, V. V. Fadeev Lomonosov Moscow State University, Faculty of Physics
Abstract:
We study the possibility of solving the multiparameter inverse problem of nonlinear laser fluorimetry of molecular systems with high local concentration of fluorophores (by the example of chlorophyll α molecules in photosynthetic organisms). The algorithms are proposed that allow determination of up to four photophysical parameters of chlorophyll a from the experimental fluorescence saturation curves. The uniqueness and stability of the inverse problem solution obtained using the proposed algorithms were assessed numerically. The laser spectrometer, designed in the course of carrying out the work and aimed at nonlinear laser fluorimetry in the quasi-stationary and nonstationary excitation regimes is described. The algorithms, proposed in this paper, are tested on pure cultures of microalgae Chlorella pyrenoidosa and Chlamydomonas reinhardtii under different functional conditions.
Received: 11.01.2011 Revised: 10.03.2011
Citation:
T. S. Gostev, V. V. Fadeev, “Determination of photophysical parameters of chlorophyll α in photosynthetic organisms using the method of nonlinear laser fluorimetry”, Kvantovaya Elektronika, 41:5 (2011), 414–419 [Quantum Electron., 41:5 (2011), 414–419]
Linking options:
https://www.mathnet.ru/eng/qe14533 https://www.mathnet.ru/eng/qe/v41/i5/p414
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