|
This article is cited in 8 scientific papers (total in 8 papers)
Application of lasers and laser-optical methods in life sciences
Low-frequency four-wave mixing spectroscopy of biomolecules in aqueous solutions
A. F. Bunkin, S. M. Pershin Wave Research Center of Prokhorov General Physics Institute, Russian Academy of Sciences
Abstract:
Four-wave mixing (FWM) spectroscopy is used to detect the rotational resonances of H2O and H2O2 molecules in DNA and denatured DNA aqueous solutions in the range ±10 cm-1 with a spectral resolution of 3 GHz. It is found that the resonance contribution of the rotational transitions of these molecules increases significantly in solutions rather than in distilled water. This fact is interpreted as a manifestation of specific properties of a hydration layer at DNA—water and denatured DNA—water interfaces. Analysis of the FWM spectra shows that the concentration of H2O2 molecules in the hydration layer of the DNA solution increases by a factor of 3 after denaturation. The FWM spectra of aqueous solutions of α-chymotrypsin protein are obtained in the range ±7cm-1 at the protein concentrations between 0 and 20 mg cm-3. It is found that the hypersound velocity in the protein aqueous solution, measured by the shift of Brillouin components in the scattering spectrum, obeys a cubic dependence on the protein concentration and reaches a value of about 3000 m s-1 at 20 mg cm-3.
Received: 30.09.2010
Citation:
A. F. Bunkin, S. M. Pershin, “Low-frequency four-wave mixing spectroscopy of biomolecules in aqueous solutions”, Kvantovaya Elektronika, 40:12 (2010), 1098–1102 [Quantum Electron., 40:12 (2010), 1098–1102]
Linking options:
https://www.mathnet.ru/eng/qe14471 https://www.mathnet.ru/eng/qe/v40/i12/p1098
|
Statistics & downloads: |
Abstract page: | 256 | Full-text PDF : | 149 | References: | 32 | First page: | 1 |
|