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This article is cited in 6 scientific papers (total in 6 papers)
Fluorescent ultramicroscopy
Fibreoptic fluorescent microscopy in studying biological objects
A. N. Morozova, A. A. Lazutkinb, I. V. Turchina, V. A. Kamenskya, I. I. Fiksa, D. V. Bezryadnovb, A. A. Ivanovab, D. M. Toptunovb, K. V. Anokhinb a Institute of Applied Physics, Russian Academy of Sciences, Nizhny Novgorod
b NII of Normal Physiology of RAMS
Abstract:
The method of fluorescent microscopy is developed based on employment of a single-mode fibreoptic channel to provide high spatial resolution 3D images of large cleared biological specimens using the 488-nm excitation laser line. The transverse and axial resolution of the setup is 5 and 13 μm, respectively. The transversal sample size under investigation is up to 10 mm. The in-depth scanning range depends on the sample transparency and reaches 4 mm in the experiment. The 3D images of whole mouse organs (heart, lungs, brain) and mouse embryos obtained using autofluorescence or fluorescence of exogenous markers demonstrate a high contrast and cellular-level resolution.
Received: 19.04.2010 Revised: 08.07.2010
Citation:
A. N. Morozov, A. A. Lazutkin, I. V. Turchin, V. A. Kamensky, I. I. Fiks, D. V. Bezryadnov, A. A. Ivanova, D. M. Toptunov, K. V. Anokhin, “Fibreoptic fluorescent microscopy in studying biological objects”, Kvantovaya Elektronika, 40:9 (2010), 842–846 [Quantum Electron., 40:9 (2010), 842–846]
Linking options:
https://www.mathnet.ru/eng/qe14344 https://www.mathnet.ru/eng/qe/v40/i9/p842
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Abstract page: | 298 | Full-text PDF : | 163 | References: | 50 | First page: | 1 |
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