Laser spectroscopy technique for estimating the efficiency of photosensitisers in biological media

A fast and highly informative method is presented for estimating the photodynamic activity of photosensitisers. The method makes it possible to determine the rate of photodegradation in erythrocyte-containing biological media in nearly in vivo conditions, estimate the degree of irreversible binding of oxygen dissolved in the medium during laser irradiation in the presence of photosensitisers, and determine the nature of degradation of photosensitisers exposed to light (photobleaching).