Technology of regeneration of the bone tissue in a rotational bioreactor: modeling of the fluid flow and laser-induced fluorescence diagnostics

A technology of growing the bone tissue on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell bulk cultivation is determined for testing the method, and an optical method for diagnostics of the bone tissue evolution in the course of its growing is developed. The fluid flow in the bioreactor is numerically simulated, which allows significant simplification of the medical experiment and appropriate choice of the optimal values of the rotation frequencies and shear stresses acting on the cell material located on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component method is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophase aminoacids, structural protein (collagene), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagene increases in the course of dynamic cultivation in the samples, which testifies to effective formation of the extracellular matrix of the bone.