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Phosphorescence kinetics of singlet oxygen in HeLa cells suspension
V. P. Belika, D. M. Beltukovaa, T. N. Belyaevab, I. M. Gadzhieva, E. S. Kornilovab, I. K. Litvinovb, I. V. Semenovaa, O. S. Vasutinskiia a Ioffe Institute, St. Petersburg
b Institute of Cytology Russian Academy of Science, St. Petersburg
Abstract:
The kinetics of singlet oxygen phosphorescence in HeLa cells suspended in phosphate buffered saline (PBS) being generated under photosensitized excitation with Radachlorin photosensitizer has been observed and studied. The characteristic time of singlet oxygen generation and its lifetime in HeLa cells have been determined. The experimental data analysis demonstrated that the singlet oxygen lifetime in cells decreases as compared to that in Radachlorin solutions in PBS and in water, while the photosensitizer triplet state lifetime increases.
Keywords:
singlet oxygen, photodynamic therapy, Radachlorin photosensitizer, HeLa cells.
Received: 06.06.2019 Revised: 10.06.2019 Accepted: 10.06.2019
Citation:
V. P. Belik, D. M. Beltukova, T. N. Belyaeva, I. M. Gadzhiev, E. S. Kornilova, I. K. Litvinov, I. V. Semenova, O. S. Vasutinskii, “Phosphorescence kinetics of singlet oxygen in HeLa cells suspension”, Pisma v Zhurnal Tekhnicheskoi Fiziki, 45:18 (2019), 33–36; Tech. Phys. Lett., 45:9 (2019), 936–939
Linking options:
https://www.mathnet.ru/eng/pjtf5321 https://www.mathnet.ru/eng/pjtf/v45/i18/p33
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Abstract page: | 62 | Full-text PDF : | 13 |
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