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Pisma v Zhurnal Tekhnicheskoi Fiziki, 2019, Volume 45, Issue 22, Pages 24–27
DOI: https://doi.org/10.21883/PJTF.2019.22.48644.17980
(Mi pjtf5263)
 

This article is cited in 6 scientific papers (total in 6 papers)

The development of segmentation algorithms in holographic microscopy and tomography for determination of morphological parameters of cells

A. V. Belashova, D. A. Gorbenkoab, A. A. Zhikhorevaa, T. N. Belyaevac, E. S. Kornilovac, I. V. Semenovaa, O. S. Vasutinskiia

a Ioffe Institute, St. Petersburg
b St. Petersburg National Research University of Information Technologies, Mechanics and Optics
c Institute of Cytology Russian Academy of Science, St. Petersburg
Full-text PDF (420 kB) Citations (6)
Abstract: Algorithms of cell segmentation on two-dimensional phase images and three-dimensional distributions of a refractive index obtained by means of digital holographic microscopy and tomography are developed. The proposed algorithms are optimized for determining cell morphology characteristics including the cell volume, projection area, and surface area. A comparative analysis of the error of cell volume determination by holographic methods using the proposed cell segmentation algorithms and the standard method of confocal fluorescence microscopy has been performed.
Keywords: holographic microscopy, holographic tomography, morphological characteristics of cells, cell segmentation.
Funding agency Grant number
Ministry of Education and Science of the Russian Federation СП-2349.2019.4
A.V. Belashov gratefully acknowledges support from the Council on grants of the President of the Russian Federation for Support of Young Scientists, project no. SP-2349.2019.4.
Received: 15.07.2019
Revised: 24.07.2019
Accepted: 24.07.2019
English version:
Technical Physics Letters, 2019, Volume 45, Issue 11, Pages 1140–1143
DOI: https://doi.org/10.1134/S1063785019110191
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: A. V. Belashov, D. A. Gorbenko, A. A. Zhikhoreva, T. N. Belyaeva, E. S. Kornilova, I. V. Semenova, O. S. Vasutinskii, “The development of segmentation algorithms in holographic microscopy and tomography for determination of morphological parameters of cells”, Pisma v Zhurnal Tekhnicheskoi Fiziki, 45:22 (2019), 24–27; Tech. Phys. Lett., 45:11 (2019), 1140–1143
Citation in format AMSBIB
\Bibitem{BelGorZhi19}
\by A.~V.~Belashov, D.~A.~Gorbenko, A.~A.~Zhikhoreva, T.~N.~Belyaeva, E.~S.~Kornilova, I.~V.~Semenova, O.~S.~Vasutinskii
\paper The development of segmentation algorithms in holographic microscopy and tomography for determination of morphological parameters of cells
\jour Pisma v Zhurnal Tekhnicheskoi Fiziki
\yr 2019
\vol 45
\issue 22
\pages 24--27
\mathnet{http://mi.mathnet.ru/pjtf5263}
\crossref{https://doi.org/10.21883/PJTF.2019.22.48644.17980}
\elib{https://elibrary.ru/item.asp?id=41848480}
\transl
\jour Tech. Phys. Lett.
\yr 2019
\vol 45
\issue 11
\pages 1140--1143
\crossref{https://doi.org/10.1134/S1063785019110191}
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  • This publication is cited in the following 6 articles:
    Citing articles in Google Scholar: Russian citations, English citations
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    Pisma v Zhurnal Tekhnicheskoi Fiziki Pisma v Zhurnal Tekhnicheskoi Fiziki
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