Abstract:
The possibility of detecting the formation of immune complexes with the use of antibodies labeled with colloidal gold nanoparticles and optical spectroscopy is considered. Changes in the spectral characteristics of sols of gold nanoparticles with diameters of 15, 60, and 90 nm conjugated with immunoglobulins in the presence of especially dangerous infectious agents are recorded and analyzed. The data obtained indicate that the developed methodological approach to optical detection of immune complexes may be promising for creating immunochemical methods of identification of infectious agents.
Citation:
A. N. Spitsyn, D. V. Utkin, M. N. Kireev, N. A. Sharapova, P. S. Erokhin, V. G. Germanchuk, V. I. Kochubey, “Optical detection of immune complexes using colloidal gold nanoparticles”, Optics and Spectroscopy, 125:5 (2018), 716–720; Optics and Spectroscopy, 125:5 (2018), 810–814
This publication is cited in the following 3 articles:
M. N. Kireev, O. V. Gromova, S. V. Borisova, S. A. Vorob'eva, V. R. Vol'nikov, R. R. Salikhov, O. A. Volokh, “Biotechnological Potential of Antigens of Plague, Cholera, Tularemia and Anthrax Pathogens, Obtained at the Russian Research Institute “Microbe””, Problemy Osobo Opasnykh Infektsii, 2024, no. 2, 15
A. N. Spitsyn, D. V. Utkin, M. N. Kireev, M. V. Ovchinnikova, O. S. Kuznetsov, P. S. Erokhin, V. I. Kochubey, “Spectrophotometric characteristics of immunoglobulin conjugates for diagnostics of the agents of particularly dangerous infections”, Optics and Spectroscopy, 128:3 (2020), 417–421
N. M. Dmytrukha, O. S. Lahutina, T. Yu. Gromovoy, “Investigation of the influence of lead compounds with particles of different dispersity on human blood plasma proteins as an express method for evaluation of their safety”, Ukr. ž. probl. med. pr., 2020:3 (2020), 202