Abstract:
Diagnostic monitoring of groups of healthy patients and patients with colorectal cancer (CRC) at various stages of the development of the disease was carried out using Raman spectroscopy (CRS) and measuring the state of polarized light in the vicinity of surface plasmon resonance (SPR) observation conditions. For the diagnosis, the reaction of serum antigens with antibodies to serum M2-pyruvate kinase (M2-PK) was used near the observation conditions of SPR, for which we used the Ellips-SPEC spectral ellipsometric complex created in the Institute of Semiconductor Physics of the Siberian Branch of the Russian Academy of Sciences (ISP SB RAS). The complex has high sensitivity, accuracy and non-destructive impact on the sample under study. The intensities of the peaks at 1005, 1157 and 1520 cm−1 in the CRS spectra of CRC patients as compared with healthy individuals as well as the intensity of the equilibrium plasmon signal were significantly lower, correlating with the stage of the disease and the presence of metastases. It allows us to consider these optical methods as a promising diagnostic approaches in the diagnosis of CRC, including in the early stages of the disease.
This work was done within the program “Epidemiological Public Health Monitoring and Study on Molecular Genetic and Molecular Biological Mechanisms of Development of Common Therapeutic Diseases in Siberia to Improve Approaches to Their Diagnosis, Prevention, and Treatment”, state order no. 0324-2018-0001, reg. no. АААА-А17-117112850280-2.
Citation:
V. N. Kruchinin, M. V. Kruchinina, Ya. I. Prudnikova, E. V. Spesivtsev, S. V. Rykhlitskii, V. A. Volodin, S. V. Shekhovtsov, S. E. Pel'tek, “The use of spectral ellipsometry and Raman spectroscopy in the screening diagnosis of colorectal cancer”, Optics and Spectroscopy, 127:1 (2019), 170–176; Optics and Spectroscopy, 127:1 (2019), 170–176
\Bibitem{KruKruPru19}
\by V.~N.~Kruchinin, M.~V.~Kruchinina, Ya.~I.~Prudnikova, E.~V.~Spesivtsev, S.~V.~Rykhlitskii, V.~A.~Volodin, S.~V.~Shekhovtsov, S.~E.~Pel'tek
\paper The use of spectral ellipsometry and Raman spectroscopy in the screening diagnosis of colorectal cancer
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 1
\pages 170--176
\mathnet{http://mi.mathnet.ru/os679}
\crossref{https://doi.org/10.21883/OS.2019.07.47946.295-18}
\elib{https://elibrary.ru/item.asp?id=41130992}
\transl
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 1
\pages 170--176
\crossref{https://doi.org/10.1134/S0030400X19070142}
Linking options:
https://www.mathnet.ru/eng/os679
https://www.mathnet.ru/eng/os/v127/i1/p170
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Hemanth Noothalapati, Keita Iwasaki, Tatsuyuki Yamamoto, “Non-invasive diagnosis of colorectal cancer by Raman spectroscopy: Recent developments in liquid biopsy and endoscopy approaches”, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 258 (2021), 119818
A. A. Man'kova, O. P. Cherkasova, E. N. Lazareva, A. B. Bucharskaya, P. A. Dyachenko, Yu. V. Kistenev, D. A. Vrazhnov, V. E. Skiba, V. V. Tuchin, A. P. Shkurinov, “Study of blood serum in rats with transplanted cholangiocarcinoma using Raman spectroscopy”, Optics and Spectroscopy, 128:7 (2020), 964–971