Abstract:
Future development of the method of immersion optical clearing of biological tissues–this method is widely used in the study of the morphology and pathologies of tissues in vitro and considered promising for in vivo applications in biophysical research and medicine—requires knowledge of the details of interaction of immersion liquids with the tissue, in particular, the characteristics both of the tissue dehydration process, which is caused by the osmotic effect of the immersion liquid, and the process of diffusion of the immersion agent (IA) into the tissue. The optical properties of skin dermis, eye sclera, tendon, and many other tissues are determined by the properties of collagen bundles, abundant in these tissues. In the present work, a convenient and reliable technique for monitoring the optical properties and geometry of collagen bundles in the course of their immersion clearing in vitro, based on optical coherence tomography (OCT), is proposed. The main advantage of this technique is that it allows one to monitor changes in the geometric and optical properties of the tissue simultaneously, without interrupting the natural course of the immersion clearing process, and to obtain reliable estimates of the characteristic times and rates of both the process of tissue dehydration and process of diffusion of the IA into the tissue.
This work was supported by the Russian Federation for Basic Research (project nos. 17-00-00275 (17-00-00272), 17-00-00270/17, and 18-52-16025/18) and by the Ministry of Education and Science of the Russian Federation (project no. 3.1586.2017/4.6).
Citation:
M. E. Shvachkina, D. D. Yakovlev, E. N. Lazareva, A. B. Pravdin, D. A. Yakovlev, “Monitoring of the process of immersion optical clearing of collagen bundles using optical coherence tomography”, Optics and Spectroscopy, 127:2 (2019), 337–346; Optics and Spectroscopy, 127:2 (2019), 359–367
\Bibitem{ShvYakLaz19}
\by M.~E.~Shvachkina, D.~D.~Yakovlev, E.~N.~Lazareva, A.~B.~Pravdin, D.~A.~Yakovlev
\paper Monitoring of the process of immersion optical clearing of collagen bundles using optical coherence tomography
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 2
\pages 337--346
\mathnet{http://mi.mathnet.ru/os653}
\crossref{https://doi.org/10.21883/OS.2019.08.48052.302-18}
\elib{https://elibrary.ru/item.asp?id=41131025}
\transl
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 2
\pages 359--367
\crossref{https://doi.org/10.1134/S0030400X19080241}
Linking options:
https://www.mathnet.ru/eng/os653
https://www.mathnet.ru/eng/os/v127/i2/p337
This publication is cited in the following 3 articles:
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Ali Jaafar, Malik H. Mahmood, Roman Holomb, László Himics, Tamás Váczi, Anton Y. Sdobnov, Valery V. Tuchin, Miklós Veres, “Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution”, J. Innov. Opt. Health Sci., 14:05 (2021)
I. Yu. Yanina, J. Schleusener, J. Lademann, V. V. Tuchin, M. E. Darvin, “The effectiveness of glycerol solutions for optical clearing of the intact skin as measured by confocal Raman microspectroscopy”, Optics and Spectroscopy, 128:6 (2020), 759–765
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