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Optics and Spectroscopy, 2019, Volume 127, Issue 4, Pages 697–703
DOI: https://doi.org/10.21883/OS.2019.10.48381.99-19
(Mi os598)
 

This article is cited in 1 scientific paper (total in 1 paper)

Biophotonics

Femtosecond absorption spectroscopy of reduced and oxidized forms of cytochrome c oxidase: excited states and relaxation processes in heme $a$ and $a_3$ centers

I. V. Shelaeva, F. E. Gosteva, T. V. Vygodinab, S. V. Lepeshkevichc, B. M. Dzhagarovc

a Semenov Institute of Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia
b Belozerski Institute of Physicochemical Biology, Moscow State University, 119991, Moscow, Russia
c Stepanov Institute of Physics, National Academy of Sciences of Belarus, 220072, Minsk, Belarus
Citations (1)
Abstract: Excited electronic states and intraheme relaxation processes in the oxidized and reduced forms of mitochondrial cytochrome c oxidase extracted from a beef heart have been investigated by femtosecond absorption spectroscopy. The spectral and kinetic characteristics of short-lived intermediates have been measured from 80 fs to 20 ps after the photoexcitation. It is found that nonradiative electronic relaxation of the excitation energy in heme $a$, both in the oxidized (Fe(III)$a$) and reduced (Fe(II)$a$) forms, occurs successively as three processes, after the end of which heme $a$ is in the ground state with a large store of vibrational energy. The subsequent vibrational relaxation (heme cooling) lasts for several picoseconds. It is found for reduced heme $a_3$ (Fe(II) $a_3$) that the electronic relaxation occurs as a result of two successive stages, which changes to vibrational relaxation in the ground state. The mechanism and dynamics of electronic excitation energy conversion in cytochrome c oxidase are analyzed.
Keywords: cytochrome c oxidase, femtosecond absorption spectroscopy, excited electronic states, relaxation processes, spectral intermediates.
Funding agency Grant number
State Assignment of the Republic of Belarus
Russian Foundation for Basic Research 17-04-00160a
Ministry of Science and Higher Education of the Russian Federation 0082-2019-0001
This study was supported by the State Research Program “Photonics and Opto- and Microelectronics 1.4.01” (2016–2020) of the Republic of Belarus, Russian Foundation for Basic Research (project no. 17-04-00160a), and a grant for the Semenov Institute of Chemical Physics, Russian Academy of Sciences, within state contract 0082-2019-0001 (registration no. AAAA-A19-119012890064-7).
Received: 13.03.2019
Revised: 13.03.2019
Accepted: 11.06.2019
English version:
Optics and Spectroscopy, 2019, Volume 127, Issue 4, Pages 756–762
DOI: https://doi.org/10.1134/S0030400X19100278
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: I. V. Shelaev, F. E. Gostev, T. V. Vygodina, S. V. Lepeshkevich, B. M. Dzhagarov, “Femtosecond absorption spectroscopy of reduced and oxidized forms of cytochrome c oxidase: excited states and relaxation processes in heme $a$ and $a_3$ centers”, Optics and Spectroscopy, 127:4 (2019), 697–703; Optics and Spectroscopy, 127:4 (2019), 756–762
Citation in format AMSBIB
\Bibitem{SheGosVyg19}
\by I.~V.~Shelaev, F.~E.~Gostev, T.~V.~Vygodina, S.~V.~Lepeshkevich, B.~M.~Dzhagarov
\paper Femtosecond absorption spectroscopy of reduced and oxidized forms of cytochrome c oxidase: excited states and relaxation processes in heme $a$ and $a_3$ centers
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 4
\pages 697--703
\mathnet{http://mi.mathnet.ru/os598}
\crossref{https://doi.org/10.21883/OS.2019.10.48381.99-19}
\elib{https://elibrary.ru/item.asp?id=41848301}
\transl
\jour Optics and Spectroscopy
\yr 2019
\vol 127
\issue 4
\pages 756--762
\crossref{https://doi.org/10.1134/S0030400X19100278}
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  • This publication is cited in the following 1 articles:
    Citing articles in Google Scholar: Russian citations, English citations
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