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Optics and Spectroscopy, 2021, Volume 129, Issue 3, Pages 357–361
DOI: https://doi.org/10.21883/OS.2021.03.50663.219-20 (Mi os180) 		 
Applied optics

Measurement of dispersion of the refractive index of microscopic volumes of the bsa aqueous solution using an interference microscope

G. V. Maksimova, A. D. Ivanova, A. A. Samoilenkob, A. A. Golopolosovc, G. G. Levinb

a National University of Science and Technology «MISIS», Moscow
b The All Russian Scientific-Research Institute for Optic Physical Metrology of Government Standard, Moscow
c Moscow State University of Geodesy and Cartography
Full-text PDF (317 kB)
DOI: https://doi.org/10.21883/OS.2021.03.50663.219-20
Abstract: An interference microscopy-based method for measuring the refractive index of microscopic objects is considered. It is shown that of special interest is the analysis of the dispersion of the refractive index of small objects of biological fluids, which can make it possible to separate fluctuations of the size and the refractive index of the object in studying living cells by methods of phase microscopy. A method for measuring the dispersion of the refractive index of microscopic volumes of fluids using the MIA-1D automated interference microscope and a measuring cell with a microwell is described. An algorithm for processing the obtained images with the aim of calculating the refractive index is presented. The measurement results for the dispersion of the refractive index of bovine serum albumin are given. It is shown that the proposed method allows one to measure the refractive index of f luids in microscopic volumes up to four or five decimal places.
Keywords: refractometry, refractive index, laser interference microscopy, albumin.
Funding agency Grant number
Russian Science Foundation 19-79-30062
The study was supported by the Russian Science Foundation, project no. 19-79-30062.
Received: 21.08.2020
Revised: 05.11.2020
Accepted: 10.11.2020
English version:
Optics and Spectroscopy, 2021, Volume 129, Issue 3, Pages 347–351
DOI: https://doi.org/10.1134/S0030400X21030103
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: G. V. Maksimov, A. D. Ivanov, A. A. Samoilenko, A. A. Golopolosov, G. G. Levin, “Measurement of dispersion of the refractive index of microscopic volumes of the bsa aqueous solution using an interference microscope”, Optics and Spectroscopy, 129:3 (2021), 357–361; Optics and Spectroscopy, 129:3 (2021), 347–351
Citation in format AMSBIB
\Bibitem{MakIvaSam21}
\by G.~V.~Maksimov, A.~D.~Ivanov, A.~A.~Samoilenko, A.~A.~Golopolosov, G.~G.~Levin
\paper Measurement of dispersion of the refractive index of microscopic volumes of the bsa aqueous solution using an interference microscope
\jour Optics and Spectroscopy
\yr 2021
\vol 129
\issue 3
\pages 357--361
\mathnet{http://mi.mathnet.ru/os180}
\crossref{https://doi.org/10.21883/OS.2021.03.50663.219-20}
\elib{https://elibrary.ru/item.asp?id=46486232}
\transl
\jour Optics and Spectroscopy
\yr 2021
\vol 129
\issue 3
\pages 347--351
\crossref{https://doi.org/10.1134/S0030400X21030103}
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