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CHEMISTRY AND MATERIAL SCIENCE
Cellular uptake of FITC-labeled Ce$_{0.8}$Gd$_{0.2}$O$_{2-x}$ nanoparticles in 2D and 3D mesenchymal stem cell systems
Danil D. Kolmanovicha, Nikita N. Chukavinab, N. A. Pivovarova, Sergey A. Khaustovb, Vladimir K. Ivanovc, Anton L. Popovab a Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, Russia
b Scientific and Educational Center, State University of Education, Moscow, Russia
c Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences, Moscow, Russia
Abstract:
Cerium-containing nanoparticles have recently been identified as promising nanozymes for advanced biomedical applications. Additional modification of the core or the surface of CeO$_2$ nanoparticles (CeO$_2$ NPs) provides them with new functionalities, making them a unique theranostic agent. In this study, dextran-stabilized CeO$_2$ NPs doped with Gd (Ce$_{0.8}$Gd$_{0.2}$O$_{2-x}$) were synthesized and further functionalized with fluorescein isothiocyanate (FITC). The synthesized nanoparticles have a high degree of biocompatibility at concentrations up to 5 mg/mL and are readily internalized by human mesenchymal stem cells cultured both in monolayers (2D system) and cellular spheroids (3D system). The functionalization of CeO$_2$ NPs with Gd and FITC dye allows for monitoring their accumulation within organs and tissues using both magnetic resonance imaging (MRI) and fluorescence spectroscopy techniques.
Keywords:
nanoparticles, 3D cell spheroid, cerium, gadolinium.
Received: 23.01.2024 Revised: 23.03.2024 Accepted: 26.04.2024
Citation:
Danil D. Kolmanovich, Nikita N. Chukavin, N. A. Pivovarov, Sergey A. Khaustov, Vladimir K. Ivanov, Anton L. Popov, “Cellular uptake of FITC-labeled Ce$_{0.8}$Gd$_{0.2}$O$_{2-x}$ nanoparticles in 2D and 3D mesenchymal stem cell systems”, Nanosystems: Physics, Chemistry, Mathematics, 15:3 (2024), 352–360
Linking options:
https://www.mathnet.ru/eng/nano1278 https://www.mathnet.ru/eng/nano/v15/i3/p352
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