R. Kh. Dzhenloda, D. G. Petrov, V. M. Shkinev, B. Ya. Spivakov, “DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection”, Mendeleev Commun., 27:3 (2017), 302

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Mendeleev Communications, 2017, Volume 27, Issue 3, Pages 302–303
DOI: https://doi.org/10.1016/j.mencom.2017.05.029 (Mi mendc1976)

This article is cited in 5 scientific papers (total in 5 papers)

Communications

DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection

R. Kh. Dzhenloda^a, D. G. Petrov^b, V. M. Shkinev^a, B. Ya. Spivakov^a

^a V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
^b Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg, Russian Federation

Full-text PDF (383 kB) Citations (5)

DOI: https://doi.org/10.1016/j.mencom.2017.05.029

Graphic abstract: DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection

Abstract: A new method is proposed for dynamic DNA extraction from environmental samples (soil, water) on suspension columns containing silica-coated magnetic sorbents (Fe₃O₄@SiO₂) under a combined influence of ultrasound and magnetic fields. The recovered DNA was subsequently determined by real-time polymerase chain reaction on an ANK-32 device. It has been shown for the first time that the suspension columns enable the preconcentration of DNA (taking Mycobacterium tuberculosis as an example) in a dynamic mode from large volumes of water and soil extracts (100 ml and more) to be performed.

Document Type: Article

Language: English

Citation: R. Kh. Dzhenloda, D. G. Petrov, V. M. Shkinev, B. Ya. Spivakov, “DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection”, Mendeleev Commun., 27:3 (2017), 302–303

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https://www.mathnet.ru/eng/mendc/v27/i3/p302

This publication is cited in the following 5 articles:

R. Kh. Dzhenloda, O. B. Mokhodoeva, T. V. Danilova, V. V. Maksimova, V. M. Shkinev, Advances in Geochemistry, Analytical Chemistry, and Planetary Sciences, 2023, 525
O. B. Mokhodoeva, V. V. Maksimova, R. Kh. Dzhenloda, V. M. Shkinev, “Magnetic Nanoparticles Modified by Ionic Liquids in Environmental Analysis”, J Anal Chem, 76:6 (2021), 675
D G Petrov, I E Antifeev, R H Djenloda, N N Germash, E D Makarova, “Application of megahertz ultrasound to isolate nucleic acids”, J. Phys.: Conf. Ser., 1697:1 (2020), 012049
P. S. Fedotov, G. I. Malofeeva, E. Yu. Savonina, B. Ya. Spivakov, “Solid-Phase Extraction of Organic Substances: Unconventional Methods and Approaches”, J Anal Chem, 74:3 (2019), 205
D G Petrov, I E Antifeev, N N Germash, E D Makarova, “Analysis of the effectiveness of the stages of the concentration of genetic material”, J. Phys.: Conf. Ser., 1400:3 (2019), 033023

Citing articles in Google Scholar: Russian citations, English citations
Related articles in Google Scholar: Russian articles, English articles

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