Abstract:
The activation energy of temperature quenching (E) of tryptophan fluorescence at pH 1–13 exhibited a maximum corresponding to the isoelectric point (pHi ≈ 6) of tryptophan at all exciting light wavelengths. The values of E nonmonotonically changed with the excitation wavelength. This behavior was explained by photoinduced electron transfer from the higher singlet-excited Sn (n>1) states of tryptophan.
Keywords:
fluorescence, tryptophan, activation energy of thermal fluorescence quenching, photoexcitation energy, pH.
Document Type:
Article
Language: English
Citation:
S. S. Ostakhov, G. A. Masyagutova, S. L. Khursan, “Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence”, Mendeleev Commun., 30:1 (2020), 59–60
Linking options:
https://www.mathnet.ru/eng/mendc1100
https://www.mathnet.ru/eng/mendc/v30/i1/p59
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