Matematicheskaya Biologiya i Bioinformatika
RUS  ENG    JOURNALS   PEOPLE   ORGANISATIONS   CONFERENCES   SEMINARS   VIDEO LIBRARY   PACKAGE AMSBIB  
General information
Latest issue
Archive
Impact factor

Search papers
Search references

RSS
Latest issue
Current issues
Archive issues
What is RSS



Mat. Biolog. Bioinform.:
Year:
Volume:
Issue:
Page:
Find






Personal entry:
Login:
Password:
Save password
Enter
Forgotten password?
Register


Matematicheskaya Biologiya i Bioinformatika, 2013, Volume 8, Issue 2, Pages 432–448 (Mi mbb153)  

This article is cited in 8 scientific papers (total in 8 papers)

Bioinformatics

Mixed promoter islands as genomic regions with specific structural and functional properties

V. V. Panyukova, S. S. Kiselevbc, K. S. Shavkunovcb, I. S. Masuliscb, O. N. Ozolinebc

a Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
b Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia
c Pushchino State Institute of Natural Sciences, Moscow region, 142290, Russia
References:
Abstract: The study is aimed at unusual genomic regions of E. coli — mixed promoter islands, which possess high density of potential transcription initiation points. They were predicted by promoter finder PlatPromU, without the consideration of conservative elements, recognized by $\sigma$-subunits of RNA polymerase, thus they are supposed to interact with polymerase, which has different $\sigma$-factors. We found that the structural and functional properties of mixed promoter islands are very similar to those of promoter islands, previously revealed by the algorithm PlatProm, which was adjusted to the search for $\sigma^{70}$-specific promoters of E. coli. Thus, both types of islands are preferentially located in the regulatory regions of genes acquired by horizontal transfer. Their double helix is characterized by a higher degree of curvature and twisting, compared to normal promoters. Both types of islands have a higher capacity to form complexes with RNAP, than normal promoters, but lower ability to initiate productive transcription. This suppression can be largely explained by the advanced ability of islands to interact with the histone-like inhibitory protein H-NS. However, RNA synthesis from the promoter island associated with the gene appY, increased upon its transfer into the plasmid pET28b-eGFP and showed dependence not only from the presence of H-NS binding sites, but also from the spatial configuration of the neighboring areas. That means that conformational changes in the genome can increase the transcriptional activity from the islands, delivering the products of adjacent genes for metabolic needs of the bacterial cell.
Key words: promoter islands, DNA structure, transcription initiation, H-NS, horizontal gene transfer, bacterial evolution.
Received 23.07.2013, Published 01.09.2013
Document Type: Article
UDC: 579:252
Language: Russian
Citation: V. V. Panyukov, S. S. Kiselev, K. S. Shavkunov, I. S. Masulis, O. N. Ozoline, “Mixed promoter islands as genomic regions with specific structural and functional properties”, Mat. Biolog. Bioinform., 8:2 (2013), 432–448
Citation in format AMSBIB
\Bibitem{PanKisSha13}
\by V.~V.~Panyukov, S.~S.~Kiselev, K.~S.~Shavkunov, I.~S.~Masulis, O.~N.~Ozoline
\paper \emph{Mixed promoter islands} as genomic regions with specific structural and functional properties
\jour Mat. Biolog. Bioinform.
\yr 2013
\vol 8
\issue 2
\pages 432--448
\mathnet{http://mi.mathnet.ru/mbb153}
Linking options:
  • https://www.mathnet.ru/eng/mbb153
  • https://www.mathnet.ru/eng/mbb/v8/i2/p432
  • This publication is cited in the following 8 articles:
    Citing articles in Google Scholar: Russian citations, English citations
    Related articles in Google Scholar: Russian articles, English articles
     
      Contact us:
     Terms of Use  Registration to the website  Logotypes © Steklov Mathematical Institute RAS, 2024