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This article is cited in 1 scientific paper (total in 1 paper)
Instrument development and devices for practical applications
The single cells and cell populations viability estimation in vitro by the time-domain impedance spectroscopy
D. D. Stupinab a Alferov Federal State Budgetary Institution of Higher Education and Science Saint Petersburg National Research Academic University of the Russian Academy of Sciences, St. Petersburg
b St. Petersburg National Research University of Information Technologies, Mechanics and Optics
Abstract:
In the present study, we investigate the sensitivity and applicability of the time-domain electrical impedance spectroscopy (EIS) techniques, namely Fourier-EIS and adaptive filtering based EIS (AF-EIS), for studying cells' populations and single living cells in natural physiological environment in vitro. Using ultra-violet radiation for decreasing cell life-span we demonstrate the possibility to distinguish the living cells' population from the dead cells by both Fourier-EIS and AF-EIS. However, determining the viability of the single cell requires significant sensitivity, which is inaccessible with the conventional Fourier-EIS contrary to AF-EIS. The latter result stems from the high noise immunity of the AF-EIS, which also makes it possible to provide the measurements using the safe for cells 15-mV excitation voltage and 10–100 nA current response. The developed single-cell AF-EIS approach opens a direct roadmap for creating accurate, robust, and easy to implement toxin and radiation hazard sensors with the living cell as an acting element and proposes a solution for actual ecological and healthcare problems.
Keywords:
Electrical Impedance Spectroscopy (EIS), Excitation Voltage Source, Adaptive Filtering, Electric Cell-substrate Impedance Sensing (ECIS), Negligible Statistical Error.
Received: 18.01.2018
Citation:
D. D. Stupin, “The single cells and cell populations viability estimation in vitro by the time-domain impedance spectroscopy”, Zhurnal Tekhnicheskoi Fiziki, 88:9 (2018), 1427–1432; Tech. Phys., 63:9 (2018), 1384–1389
Linking options:
https://www.mathnet.ru/eng/jtf5829 https://www.mathnet.ru/eng/jtf/v88/i9/p1427
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