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Zhurnal Tekhnicheskoi Fiziki, 2018, Volume 88, Issue 9, Pages 1404–1406
DOI: https://doi.org/10.21883/JTF.2018.09.46428.2610 (Mi jtf5825) 		 
Instrument development and devices for practical applications

Microchip devices for nucleic acid amplification

Ya. S. Posmitnayaab, A. L. Bul'anitsaac, A. N. Tupika, G. E. Rudnitskayaa, N. A. Esikovaa, T. A. Lukashenkoa, A. I. Tsymbalova, A. A. Evstrapovab

a Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
b St. Petersburg National Research University of Information Technologies, Mechanics and Optics
c Peter the Great St. Petersburg Polytechnic University
Full-text PDF (430 kB)
DOI: https://doi.org/10.21883/JTF.2018.09.46428.2610
Abstract: Basic requirements for microchip devices used in nucleic acid amplification by means of a polymerase chain reaction (PCR), as well as in their isothermal amplification, are considered, and the results of testing the devices made of various materials are reported. Tests involved detecting a small amount of nucleic acid molecules in a sample by the colony PCR technique. The uniformity of the molecular colony distribution over the reaction chamber of the device has been quantitatively estimated. The application potential of the microchips for molecular diagnostics is discussed.
Keywords: Microchip Device, Nucleic Acid Amplification, Loop-mediated Isothermal Amplification, Molecular Colonies, Helicase-dependent Amplification.
Received: 24.12.2017
English version:
Technical Physics, 2018, Volume 63, Issue 9, Pages 1361–1363
DOI: https://doi.org/10.1134/S1063784218090165
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: Ya. S. Posmitnaya, A. L. Bul'anitsa, A. N. Tupik, G. E. Rudnitskaya, N. A. Esikova, T. A. Lukashenko, A. I. Tsymbalov, A. A. Evstrapov, “Microchip devices for nucleic acid amplification”, Zhurnal Tekhnicheskoi Fiziki, 88:9 (2018), 1404–1406; Tech. Phys., 63:9 (2018), 1361–1363
Citation in format AMSBIB
\Bibitem{PosBulTup18}
\by Ya.~S.~Posmitnaya, A.~L.~Bul'anitsa, A.~N.~Tupik, G.~E.~Rudnitskaya, N.~A.~Esikova, T.~A.~Lukashenko, A.~I.~Tsymbalov, A.~A.~Evstrapov
\paper Microchip devices for nucleic acid amplification
\jour Zhurnal Tekhnicheskoi Fiziki
\yr 2018
\vol 88
\issue 9
\pages 1404--1406
\mathnet{http://mi.mathnet.ru/jtf5825}
\crossref{https://doi.org/10.21883/JTF.2018.09.46428.2610}
\elib{https://elibrary.ru/item.asp?id=36904366}
\transl
\jour Tech. Phys.
\yr 2018
\vol 63
\issue 9
\pages 1361--1363
\crossref{https://doi.org/10.1134/S1063784218090165}
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