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Zhurnal Tekhnicheskoi Fiziki, 2018, Volume 88, Issue 10, Pages 1477–1481
DOI: https://doi.org/10.21883/JTF.2018.10.46489.6-18 (Mi jtf5788) 		 
This article is cited in 4 scientific papers (total in 4 papers)

Instrument development and devices for practical applications

Simulation of kinetic curves of polymerase chain reaction obtained using fluorescent oligonucleotide probes

A. A. Fedorova, D. G. Sochivkob, D. A. Varlamovc, V. E. Kurochkina

a Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
b ZAO Sintol, Moscow
c All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia
Full-text PDF (108 kB) Citations (4)
DOI: https://doi.org/10.21883/JTF.2018.10.46489.6-18
Abstract: At present, many polymerase chain reaction models have been proposed for exact quantitative estimation of the reaction results. In most models, kinetics of product accumulation and kinetics of fluorescent reporter are assumed to be identical. A model of the polymerase chain reaction is proposed to study the difference of such functions in the system in which a hybridization probe serves as the source of the detected fluorescence signal. Adequacy of the model is verified, and significant possible difference of the accumulation kinetics of product and probe reporter is demonstrated.
Keywords: Fluorescent Oligonucleotide Probes, Aggregation Kinetics, Exact Quantitative Estimates, Detected Fluorescence Signal, Complementary Chain.
Funding agency Grant number
Russian Academy of Sciences - Federal Agency for Scientific Organizations 0074-2014-001
Received: 11.01.2018
English version:
Technical Physics, 2018, Volume 63, Issue 10, Pages 1430–1434
DOI: https://doi.org/10.1134/S1063784218100109
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: A. A. Fedorov, D. G. Sochivko, D. A. Varlamov, V. E. Kurochkin, “Simulation of kinetic curves of polymerase chain reaction obtained using fluorescent oligonucleotide probes”, Zhurnal Tekhnicheskoi Fiziki, 88:10 (2018), 1477–1481; Tech. Phys., 63:10 (2018), 1430–1434
Citation in format AMSBIB
\Bibitem{FedSocVar18}
\by A.~A.~Fedorov, D.~G.~Sochivko, D.~A.~Varlamov, V.~E.~Kurochkin
\paper Simulation of kinetic curves of polymerase chain reaction obtained using fluorescent oligonucleotide probes
\jour Zhurnal Tekhnicheskoi Fiziki
\yr 2018
\vol 88
\issue 10
\pages 1477--1481
\mathnet{http://mi.mathnet.ru/jtf5788}
\crossref{https://doi.org/10.21883/JTF.2018.10.46489.6-18}
\elib{https://elibrary.ru/item.asp?id=36904475}
\transl
\jour Tech. Phys.
\yr 2018
\vol 63
\issue 10
\pages 1430--1434
\crossref{https://doi.org/10.1134/S1063784218100109}
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    • This publication is cited in the following 4 articles:
    Citing articles in Google Scholar: Russian citations, English citations
    Related articles in Google Scholar: Russian articles, English articles
    		Zhurnal Tekhnicheskoi Fiziki Zhurnal Tekhnicheskoi Fiziki
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