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Zhurnal Tekhnicheskoi Fiziki, 2020, Volume 90, Issue 9, Pages 1581–1585
DOI: https://doi.org/10.21883/JTF.2020.09.49693.422-19
(Mi jtf5217)
 

This article is cited in 3 scientific papers (total in 3 papers)

III International Conference Physics -- Life Sciences
Physical methods in photobiology

Model of fluorescent signal generation from an intercalating dye in the course of a polymerase chain reaction

A. A. Fedorova, D. G. Sochivkob, D. A. Varlamovb

a Institute for Analytical Instrumentation, Russian Academy of Sciences, St. Petersburg
b ZAO Sintol, Moscow
Full-text PDF (112 kB) Citations (3)
Abstract: A number of models of the polymerase chain reaction have been suggested to date with the aim of gaining exact quantitative estimates for reaction products. These models usually identify the reaction product kinetics with a kinetics of fluorescence reporter. Here, a model of polymerase chain reaction is suggested in which the results of the reaction are detected using an intercalating dye. Simulation data indicate a significant discrepancy between the reaction product kinetics and the fluorescent signal from a bonded intercalator.
Keywords: PCR model, PCR curve, intercalation dye.
Funding agency Grant number
Ministry of Education and Science of the Russian Federation 075-00780-19-02
This study was carried out in the framework of state order no. 075-00780-19-02 of the Ministry of Science and Higher Education of the Russian Federation, grant no. 0074-2019-0013.
Received: 19.12.2019
Revised: 25.02.2020
Accepted: 03.03.2020
English version:
Technical Physics, 2020, Volume 65, Issue 9, Pages 1516–1520
DOI: https://doi.org/10.1134/S1063784220090169
Bibliographic databases:
Document Type: Article
Language: Russian
Citation: A. A. Fedorov, D. G. Sochivko, D. A. Varlamov, “Model of fluorescent signal generation from an intercalating dye in the course of a polymerase chain reaction”, Zhurnal Tekhnicheskoi Fiziki, 90:9 (2020), 1581–1585; Tech. Phys., 65:9 (2020), 1516–1520
Citation in format AMSBIB
\Bibitem{FedSocVar20}
\by A.~A.~Fedorov, D.~G.~Sochivko, D.~A.~Varlamov
\paper Model of fluorescent signal generation from an intercalating dye in the course of a polymerase chain reaction
\jour Zhurnal Tekhnicheskoi Fiziki
\yr 2020
\vol 90
\issue 9
\pages 1581--1585
\mathnet{http://mi.mathnet.ru/jtf5217}
\crossref{https://doi.org/10.21883/JTF.2020.09.49693.422-19}
\elib{https://elibrary.ru/item.asp?id=44154280}
\transl
\jour Tech. Phys.
\yr 2020
\vol 65
\issue 9
\pages 1516--1520
\crossref{https://doi.org/10.1134/S1063784220090169}
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  • This publication is cited in the following 3 articles:
    Citing articles in Google Scholar: Russian citations, English citations
    Related articles in Google Scholar: Russian articles, English articles
    Zhurnal Tekhnicheskoi Fiziki Zhurnal Tekhnicheskoi Fiziki
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