Optical clearing of human eye sclera by aqueous 30%-glucose solution

In this work, optical clearing of human eye sclera in vitro under action of aqueous 30% glucose solution was studied. It was shown that diffusion of glucose in tissue results in significant decrease of light scattering, which manifests itself in both decreasing reflectance and increasing transmittance of the samples. At that, absorption coefficient does not change. Measurements were performed with spectrophotometer CARY-2415 in the spectral range from 400 to 800 nm by a standard method. Inverse Adding-Doubling Method was used for processing of data of the spectral measurements and reconstruction of optical parameters. Obtained results can be used in the development of new methods and optimization of existing ones in optical diagnostics and therapy of eye diseases.