|
This article is cited in 1 scientific paper (total in 1 paper)
ANALYSIS AND MODELING OF COMPLEX LIVING SYSTEMS
Fluorescent probe immobilization into enzyme molecules
V. S. Maryakhina, V. V. Gunkov Orenburg State University, Pobedy pr, Orenburg, 460018, Russia
Abstract:
The results of the experimental and theoretical researches of kinetics of erythrosine penetration into collagenase molecules have represented in this paper. The case with introduction of the compound (fluorescent probe) which has dimers to enzyme solution as an injection has been considered. It was shown that monomers and dimers can penetrate into enzyme molecules with formation complexes monomer-enzyme, dimer-enzyme. Moreover, transformation of probe fluorescence spectra is at each time moment. Spectrum maximum shift, and its form change. At a time, the immobilized dye dimers greatly impact to formation of end fluorescence spectrum. Well correlation between experimental and theoretical results confirms reality of the obtained data.
Keywords:
diffusion, molecular probe, enzyme, collagenase, fluorescent diagnostics.
Received: 06.04.2013 Revised: 20.09.2013
Citation:
V. S. Maryakhina, V. V. Gunkov, “Fluorescent probe immobilization into enzyme molecules”, Computer Research and Modeling, 5:5 (2013), 835–843
Linking options:
https://www.mathnet.ru/eng/crm439 https://www.mathnet.ru/eng/crm/v5/i5/p835
|
Statistics & downloads: |
Abstract page: | 66 | Full-text PDF : | 17 | References: | 22 |
|