Abstract:
Here we demonstrate the results of kinetic modeilng of DNA double-strand breaks induction and repair and phosphorilated histone H2AX (γ−H2AX) and Rad51 foci formation in primary human fibroblasts exposed to low-LET ionizing radiation (IR). The model describes two major paths of DNA double-strand breaks repair: non-homologous end joining (NHEJ) and homologous recombination (HR) and considers interactions between DNA and several repair proteins (DNA-PKcs, ATM, Ku70/80, XRCC1, XRCC4, Rad51, RPA, etc.) using mass action equations and Michaelis-Menten kinetics. Experimental data on DNA rejoining kinetics and γ−H2AX and Rad51 foci formation in vicinity of double strand breaks in primary human fibroblasts exposed to low-LET IR with various dose rates and exposure times was utilized for training and statistical validation of the model.
Keywords:
DNA double-strand breaks, DNA repair, kinetic modeling, low-LET ionizing radiation, dose rate.
Received: 04.02.2015
Document Type:
Article
UDC:
577.31
Language: Russian
Citation:
I. V. Ozerov, A. N. Osipov, “Kinetic model of dna double-strand break repair in primary human fibroblasts exposed to low-let irradiation with various dose rates”, Computer Research and Modeling, 7:1 (2015), 159–176
\Bibitem{OzeOsi15}
\by I.~V.~Ozerov, A.~N.~Osipov
\paper Kinetic model of dna double-strand break repair in primary human fibroblasts exposed to low-let irradiation with various dose rates
\jour Computer Research and Modeling
\yr 2015
\vol 7
\issue 1
\pages 159--176
\mathnet{http://mi.mathnet.ru/crm176}
\crossref{https://doi.org/10.20537/2076-7633-2015-7-1-159-176}
Linking options:
https://www.mathnet.ru/eng/crm176
https://www.mathnet.ru/eng/crm/v7/i1/p159
This publication is cited in the following 3 articles:
O. P. Gundarova, V. P. Fedorov, A. G. Kvaratskheliya, V. N. Maslov, “Radiation-Induced Changes in Protein Content in the Brain Neurons”, Žurnal anatomii i gistopatologii, 9:2 (2020), 17
G. S. Ritter, V. P. Nikolin, N. A. Popova, A. S. Proskurina, P. E. Kisaretova, O. S. Taranov, T. D. Dubatolova, E. V. Dolgova, E. A. Potter, S. S. Kirikovich, Y. R. Efremov, S. I. Bayborodin, M. V. Romanenko, M. I. Meschaninova, A. G. Venyaminova, N. A. Kolchanov, S. S. Bogachev, “Characteristic of the active substance of the Saccharomyces cerevisiae preparation having radioprotective properties”, Vestn. VOGiS, 24:6 (2020), 643
N. Vorobeva, N. Vorob'eva, V. Uiba, V. Uyba, O. Kochetkov, O. Kochetkov, T. Astrelina, T. Astrelina, M. Pustovalova, M. Pustovalova, A. Grekhova, A. Grehova, T. Blokhina, T. Blohina, E. Yashkina, E. Yashkina, D. Kabanov, D. Kabanov, V. Nikitina, V. Nikitina, E. Suchkova, E. Suchkova, I. Kobzeva, I. Kobzeva, A. Osipov, A. Osipov, “3H-Thymidine Influence on DNA Double Strand Breaks Induction in Cultured Human Mesenchymal Stem Cells”, Medical Radiology and radiation safety, 63:1 (2018), 28
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